《植物生理学报》 2014, 50(12): 1840-1844

高温对水稻胚乳淀粉合成关键酶活性及内源激素含量的影响

张桂莲^1,2, 廖斌¹, 武小金², 肖应辉¹, 肖浪涛^3,*, 陈立云^1,*

^1,# 湖南农业大学农学院, 长沙410128; ^2,# 深圳市龙岗区农业科技推广中心博士后创新基地, 广东深圳518118; ³湖南农业大学植物激素与生长发育湖南省重点实验室, 长沙410128

通信作者：肖浪涛；E-mail: langtaoxiao@163.com, chenliyun996@163.com；Tel: 0731-84635261, 0731-84618757

摘　要：

以耐热性不同的两个水稻品系为材料, 研究了高温胁迫对胚乳淀粉合成关键酶活性及内源激素含量的影响。结果表明, 高温胁迫下胚乳ADPG焦磷酸化酶活性(AGPase)、可溶性淀粉合成酶(SSS)和淀粉分支酶(SBE)活性升高, 到达峰值后下降。耐热品系996胚乳淀粉合成酶类活性的下降幅度小于热敏感品系4628。高温下淀粉积累速率与AGPase、SSS活性显著相关。高温下胚乳中ABA含量升高, IAA含量和Z+ZR含量降低, 热敏感品系4628胚乳中内源激素含量变化幅度均大于耐热品系996。

关键词：水稻; 高温; 胚乳; 淀粉合成关键酶; 激素

收稿：2014-08-12   修定：2014-11-22

资助：国家自然科学基金项目(30900874)、湖南省自然科学基金项目(11JJ3026)、湖南省教育厅优秀青年基金项目(13B052)和作物学开放基金项目(ZWKF201301)。 # 同等贡献。

Effect of High Temperature on Activities of Enzymes Associated with Starch Synthesis and Hormones Contents in Endosperm of Rice

ZHANG Gui-Lian^1,2, LIAO Bin¹, WU Xiao-Jin², XIAO Ying-Hui¹, XIAO Lang-Tao^3,*, CHEN Li-Yun^1,*

^1,# College of Agronomy, Hunan Agricultural University, Changsha 410128, China; ^2,# Post-Doctoral Innovation Base of Agricultural Science and Technology Promotion Center of Longgang District, Shenzhen, Guangdong 518118, China; ³Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China

Corresponding author: XIAO Lang-Tao; E-mail: langtaoxiao@163.com, chenliyun996@163.com; Tel: 0731-84635261, 0731-84618757

Abstract:

The experiment was carried out in growth chamber to investigate the effect of high temperature on the activities of the key enzymes associated with starch synthesis and hormone contents in endosperm of two rice (Oryza sativa) lines including the heat-tolerant line 996 and heat-sensitive line 4628. The results showed that AGPase, SSS and SBE enzyme activities increased under high temperature stress, then decreased after the period of the peak, the decreasing extent in heat resistant line 996 was lower than that in heat sensitive line 4628. There was a significant correlation between the starch accumulation rate and the AGPase, SSS activities. The ABA content increased, IAA content and Z+ZR content decreased under high temperature stress, the change scope of endogenous hormone content in heat-tolerant line 996 was greater than that of heat-sensitive line 4628.

Key words: rice (Oryza sativa); high temperature; endosperm; key enzymes of starch synthesis; hormone